بررسی ایمنوهیستوشیمی وضعیت گیرنده های استروژن و پروژسترون و نشانگر تکثیر سلولی (Ki-67) در سرطان پستان

Background and Aim: Breast carcinoma is a pervasive malignancy in the world, and in our country-Iran-as well. Extensive studies have been done on its diagnostic and therapeutic procedures and factors affecting its prognosis. The present study aimed at determining proliferative rate of cells, the condition of steroid hormone receptors, correlation of these with each other and with other factors such as age ,lymph nodes involvement, and tumoral grade in breast carcinoma. Materials and Methods: This analytical and applied study was carried out in the pathology laboratory of Ghaem hospital -affiliated to Mashhad University of Medical Sciences- in 2004. Fifty cases of breast carcinoma, confirmed through histopathology, were selected. After checking microscopic slides and preparing suitable sections, specific staining through immunohistochemical procedure with estrogen (ER), progesteron (PR) markers, and Ki-67 was done. Then the slides were examined by two pathologists to determine immunoreactivity of tumoral cells by means of H-score system for ER and PR, the percent of tumoral cells for Ki- 67, and then the results were reported. The obtained data was analysed at the significant level P≤0.05 applying ANOVA and Spearman correlation coefficient statistical tests. Results: Ki-67, ER and PR expression in patients with invasive ductal carcinoma were 24.25%, 89.62 and 59 respectively. Between Ki-67 and ER no relationship was found. But there was a significant correlation between Ki-67 and PR (P=0.05). Also, there was a significant correlation between the incidence of ER and PR (P=0.00). Ki-67 expression in the three grades of invasive ductal breast carcinoma revealed distinct and meaningful difference (P=0.01). Among rare types of breast carcinomas, the most expression of Ki-67, PR and ER was observed in invasive lobular carcinoma and the least in papillary and medulary carcinomas. Conclusion: Immunohistochemistry is very important in the prognosis and treatment of breast carcinoma. Thus proper procedures, standardization of ER and PR is necessary.